Development of Cryopreservation and Thawing Recovery Materials
for 3D Cell Therapeutics and Organoid-Based Therapies
To develop 3D cell survival after freezing and thawing, we designed a hybrid cryoprotective platform incorporating genetically engineered recombinant antifreeze proteins and antioxidants into lipid, gelatin, and hydrogel-based systems

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Designing a three-dimensional cell cryoprotective platform using recombinant antifreeze proteins and antioxidants
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Developing a multi-modal delivery system based on lipid membranes, cell-derived membranes, gelatin microparticles, and hydrogels
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Establishing three-dimensional cell protection strategies encompassing cellular penetration, co-culture, and encapsulation
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Constructing an evaluation framework to assess cell viability and structural integrity before and after freezing and thawing
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Proposing a hybrid cryoprotective technology for next-generation storage and transport of three-dimensional cell systems

